The enzyme-linked immunosorbent assay (ELISA) is a technique for detecting antigens in biological material. ELISA, like other immunoassays that use antibodies to identify a target antibody, detects the antigen through highly precise antibody-antigen interactions. You can know more about ELISA testing services via https://www.bosterbio.com/services/assay-services/elisa-testing-service.
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An ELISA test uses the immobilization of the antigen to a solid surface. This can be done directly or by using a captured antibody that is immobilized on the surface.
The antigen is then combined with a detection antibody and a molecule that can be detected, such as an enzyme or fluorophore.
ELISA tests are typically done in a multi-well plate (96 or 384 wells), which provides the solid surface for immobilizing the antigen. The immobilization of analytes allows for the separation of antigen from other components of the sample.
This makes ELISA one the most convenient assays for multiple samples at once. An ELISA is a simple method of testing antigens.
- High sensitivity, specificity and specificity. It is not uncommon for ELISAs detect antigens down to the picogram level using antibodies.
- High throughput: Commercial ELISA kits come in a 96-well format. However, the assay can easily be adapted for use on 384-well plates.
- Simple to use: The protocols are simple to follow and require little hand-holding.
- Quantitative can be used to determine the antigen concentration in a sample.
- Ability to test various samples: plasma, serum, cellular, and tissue extracts and urine among others.